鳄鱼血SDS-PAGE鉴别方法的研究

目的: 建立鳄鱼血SDS-PAGE鉴别方法。 方法: 利用电泳缓冲液制备鳄鱼血、绵羊血、豚鼠血、鸡血、兔血、鹿血等6种动物全血样品液,采用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)法,对6种动物全血进行鉴别研究。 结果: 以7.5%~11%的梯度分离胶、60 μg蛋白量上样,大板(16 cm×20 cm)进行SDS-PAGE鉴别,6种动物全血的电泳谱带有明显差异,鳄鱼血存在特征条带。 结论: 本方法灵敏度高、专属性强,可作为鳄鱼血与其他动物血的鉴别依据。

Objective: To establish the SDS-PAGE identification method for crocodile blood. Methods: Electrophoresis buffer was used to prepare six animals' whole blood sample solution.The animals were:crocodile,sheep,guinea pig,chicken,rabbit,and deer.Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) method was used to identify six animals' whole blood. Results: The best SDS-PAGE conditions to get good resolution were 60 μg protein sample loaded to each well and using the 7.5%-11% gradient separating gel plate (16 cm×20 cm) to run the samples.The obviously different electrophoresis bands of six animals' whole blood were obtained by the method and crocodile blood has the characteristic bands. Conclusion: This method is sensitive and could be used easily to distinguish the crocodile blood from other five kinds bleed.