双波长RP-HPLC法同时测定牛黄上清片中栀子苷和黄芩苷的含量

目的: 建立双波长RP-HPLC法同时测定牛黄上清片中黄芩苷和栀子苷含量的方法。 方法: 色谱柱:Hibor C₁₈柱(4.6 mm×150 mm,5 μm),流动相:乙腈-0.2%磷酸水溶液,采用梯度洗脱,检测波长:240 nm(栀子苷),278 nm(黄芩苷),柱温:30 ℃。 结果: 栀子苷和黄芩苷进样量分别在0.16~0.96 μg(r=0.9999),0.18~1.08 μg(r=0.9999)范围内线性关系良好,平均加样回收率分别为95.6%,96.0%,RSD分别为2.7%,2.5%。 结论: 该法简便、快速、灵敏,可用于牛黄上清片中栀子苷和黄芩苷的含量测定。

Objective: To develop a double wavelength RP-HPLC method for simultaneous determination of baicalin and geniposide in Niuhuang Shangqing tablets. Method: The analysis was performed on a Hibor C₁₈ column (150 mm×4.6 mm,5 μm)with gradient elution of acetonitrile-0.2% phosphoric acid solution.Geniposide and baicalin were detected at 240 nm and 278 nm respectively.The column temperature was 30 ℃. Results: The linear range was 0.16-0.96 μg (r=0.9999) for geniposide and 0.18-1.08 μg (r=0.9999) for baicalin.The average recoveries of geniposide and baicalin were 95.6% and 96.0% respectively,RSD were 2.7% and 2.5%. Conclusion: The method is simple,accurate and sensitive and can be used for simultaneously determining the contents of geniposide and baicalin in Niuhuang Shangqing tablets.