SPE-UPLC法测定养血清脑颗粒中的芍药苷含量

目的: 采用SPE-UPLC法分析养血清脑颗粒中芍药苷的含量。 方法: 采用C₁₈固相萃取柱去除养血清脑颗粒中的基质,超高效液相色谱法测定芍药苷含量。采用Acquity BEH C₁₈(50 mm×2.1 mm,1.7 μm)色谱柱,乙腈-水(25∶ 75)为流动相,检测波长为230 nm,流速为0.2 mL·min^-1,柱温40 ℃。 结果: SPE-UPLC法测得芍药苷浓度在5.0~80.0 μg·mL^-1范围内与其峰面积线性关系良好(r=0.9999),平均回收率(n=6)为99.4%(RSD=2.83%)。 结论: 该方法准确、简便并能有效去除药物基质保护色谱柱,可用于该制剂的质量控制。

Objective: To establish a SPE-UPLC method for the determination of peoniflorin in Yangxue Qingnao granula. Methods: The sample was purified with C₁₈ solid phase column and its content was determined with UPLC method.The UPLC column used in the experiment was C₁₈(50 mm×2.1 mm,1.7 μm),and the mobile phase was acetonitrile-water(25∶ 75)at a flow rate of 1.0 mL·min^-1;The detection wavelength was 230 nm,and the experiment was accomplished at 40 ℃. Results: The content of peoniflorin determined by SPE-UPLC method showed good linear relationship with the peak areas when their concentrations at 5.0-80·0 μg·mL^-1(r=0.9999),the average recovery(n=6)of peoniflorin was 99.4%(RSD=2.83%). Conclusion: The method is simple,accurate,effective to reduce the damage of base material to the chromatographic column and can be used to control the quality of Yangxue Qingnao granula.