高效液相色谱-二极管阵列检测器法测定扛板归不同部位中齐墩果酸和熊果酸的含量

目的: 建立测定扛板归不同部位中齐墩果酸和熊果酸含量的高效液相色谱-二极管阵列检测器方法。方法: 色谱柱为Kromasil C₁₈柱(4.6 mm×250 mm,5 μm),流动相为0.5%磷酸水溶液-甲醇(10∶[KG-*2]90),流速0.8 mL·min^-1,检测波长210 nm,柱温30 ℃。结果: 齐墩果酸在0.228~2.850 μg时,与峰面积呈良好的线性关系(r=0.9998),平均回收率为 97.4%,RSD为1.5%(n=6);熊果酸进样量在0.544~6.800 μg时,与峰面积呈良好的线性关系(r=0.9998),平均回收率为 96.9%,RSD为1.2%(n=6)。结论: 方法准确,操作简便,数据可靠,可用于扛板归中齐墩果酸和熊果酸的含量测定。

Objective: To establish a HPLC-DAD method for the determination of oleanolic acid and ursolic acid in the different parts of Polygonum perfoliatum L. Methods: Kromasil C₁₈ column(4.6 mm×250 mm,5 μm)was adopted.The mobile phase consisted of methanol-0.5% phosphoric acid aqueous solution(10∶90)at a flow rate of 0.8 mL·min^-1,and the column temperature was set at 30 ℃.The detection wavelength was 210 nm.Results: Oleanolic acid showed good relationship at the range of 0.228-2.850 μg(r=0.9998)and the recovery was 97.4% with 1.5%(n=6)of RSD.Ursolic acid showed good relationship at the range of 0.544-6.800 μg(r=0.9998)and the recovery was 96.9% with 1.2%(n=6)of RSD.Conclusion: The proposed method is accurate,simple,and promises to be applicable for the determination of oleanolic acid and ursolic acid in the different parts of P.perfoliatum L.