RP-HPLC法测定酒石酸茚喹诺啉脂质体中药物含量及包封率

目的: 建立酒石酸茚喹诺啉脂质体中药物含量及包封率测定方法。 方法: 采用逆向蒸发法制备脂质体,葡聚糖凝胶柱层析法分离脂质体和游离药物,以磷酸盐缓冲液为洗脱介质。采用反相高效液相色谱法测定脂质体中酒石酸茚喹诺啉的含量。 结果: 酒石酸茚喹诺啉含量测定方法的回收率为99.5%,在5~200 μg·mL^-1范围内线性关系良好(r=0.9998);柱层析法分离游离药物的柱回收率为101.0%,加样回收率为97.9%。样品的平均包封率为40.3%。 结论: 酒石酸茚喹诺啉脂质体中药物含量及包封率测定方法准确、灵敏,可用于测定酒石酸茚喹诺啉脂质体的包封率。

Objective: To establish an RP-HPLC method for the determination of content and entrapment efficiency of tartrate indoquinoline liposomes. Methods: The content of tartrate indoquinoline was determined by RP-HPLC.Entrapment efficiency was determined after the separation of the unentrapped drug by Sephadex-G50 chromatography. Results: A good linear relationship was found between peak area and the concentration of tartrate indoquinoline in the range of 5-200 μg·mL^-1(r=0.9998),the sample recovery was arround of 99.5%.The recovery of Sephadex-G50 chromatography was 101.0%and the recovery of tartrate indoquinoline was 97.9%.The average entrapment efficiency of tartrate indoquinoline liposome was 40.3%. Conclusion: This method is accurate,sensitive and applicable for determination of content and entrapment efficiency of tartrate indoquinoline liposome.