高效液相色谱法测定伤科缓释胶囊中士的宁和马钱子碱的含量

目的: 建立伤科缓释胶囊中士的宁和马钱子碱的含量测定方法。 方法: 采用高效液相色谱法,使用迪马ODS(5 μm,4.6 mm×200 mm)色谱柱,流动相为乙腈-含0.2%磷酸和0.2%三乙胺的水(11∶ 89),流速1 mL·min^-1,检测波长260 nm,柱温为室温。 结果: 士的宁的进样量在0.29~1.74 μg范围内线性关系良好(r=0.9999),平均加样回收率为98.4%(RSD=1.4%);马钱子碱进样量在0.245~1.47 μg范围内线性关系良好(r=0.9997),平均加样回收率为99.0%(RSD=1.6%)。 结论: 该方法简便、灵敏、准确,可用于伤科缓释胶囊的质量控制。

Objective: To establish a method for the determination of strychnine and brucine in Shangke sustained release capsules. Methods: HPLC method was adopted. The analysis was performed with ODS(5 μm, 4.6 mm×200 mm) column; The mobile phase was acetonitrile-water containing 0.2% phosphorous acid and 0.2% triethylamine (11∶ 89),at a flow rate of 1 mL·min^-1;The wavelength of the detector was 260 nm, and the column temperature was room temperature. Results: The linear ranges of strychnine, brucine were 0.29-1.74 μg (r=0.9999), 0.245-1.47 μg (r=0.9997); The average recoveries of strychnine, brucine was 98.4%, 99.0%, with the RSD of 1.4%, 1.6%, respectively. Conclusion: The method is simple, rapid and accurate, and suitable for the quality control of Shangke sustained release capsules.