HPLC-UV-ELSD测定山银花中绿原酸、灰毡毛忍冬皂苷乙和川续断皂苷乙的含量

目的: 建立同时测定传统中药山银花中绿原酸、灰毡毛忍冬皂苷乙和川续断皂苷乙含量的HPLC分析方法。 方法: 采用Phenomenex Luna C₁₈(250 mm×4.6 mm,5 μm)色谱柱,流动相0.4%醋酸(A)-乙腈(B),梯度洗脱(0~12 min,80%A;12~13 min,80%A→67%A;13~30 min,67%A→65%A;30~35 min,65%A→80%A),流速0.5 mL·min^-1;UV检测:检测绿原酸,λ=326 nm;ELSD检测:检测灰毡毛忍冬皂苷乙和川续断皂苷乙,雾化室温度88 ℃,漂移管温度108 ℃,N₂气流量1.0 L·min^-1。 结果: 线性范围内3个化合物呈良好的线性关系(R²>0.9959),加样回收率在99%~101%之间(n=6),该方法精密度、稳定性和重复性良好,RSD＜2%。 结论: 该方法能够方便、准确地测定山银花中绿原酸、灰毡毛忍冬皂苷乙和川续断皂苷乙的含量。

Objective: To establish a new analysis method for the determination of three analytes,namely chlorogenic aicd,macranthoidin B and dipsacoside B in Flos Lonicerae,a commonly used traditional Chinese medicine(TCM)herb with HPLC. Methods: Phenomenex Luna C₁₈(250 mm×4.6 mm,5 μm)column was adopted;The mobile phase consisted of 0.4% aqueous acetic acid(A)and acetonitrile(B)with a gradient elution of 80%A at 0-12 min,80%A→77%A at 12-13 min,67%A→65%A at 13-30 min and 65%A→80%A at 30-35 min,at a flow rate of 0.5 mL·min^-1.The UV detection wavelength was
[326] nm for chlorogenic aicd.The drift tube temperature of ELSD was set at
[108] ℃,with the nitrogen flow rate of 1.0 L·min^-1 for macranthoidin B and dipsacoside B. Results: Good linear regression(R²>0.9959) was within wide test ranges.The recovery rate was between 99% and 101%(n=6).This method had better precision,stability and repeatability,RSD＜2%. Conclusion :This method is convenient and accurate for determination of chlorogenic aicd,macranthoidin B and dipsacoside B in Flos Lonicerae.