RP-HPLC法测定狼毒中狼毒乙素和岩大戟内酯B的含量

目的: 建立同时测定狼毒药材中狼毒乙素和岩大戟内酯B的HPLC定量分析方法。 方法: 采用Lichrospher C₁₈ (4.6 mm× 250 mm,5 μm)色谱柱,以乙腈(A)-水(B)为流动相梯度洗脱(0~5 min,40%A;5~10 min,40%A→50%A;10~30 min,50%A→55%A;30~40 min,55%A→60%A;40~45 min,60%A→70%A),流速1.0 mL·min^-1;检测波长226 nm,柱温30 ℃。 结果: 狼毒乙素线性范围为0.005~0.863 μg(r=0.9999),平均回收率为96.4%(RSD=1.4%,n=6);岩大戟内酯B线性范围为0.003~0.496 μg(r=0.9999),平均回收率为96.0%(RSD=2.1%,n=6)。 结论: 本方法专属性强,结果准确,简便可行,可用于狼毒药材的质量控制。

Objective: To develop an HPLC method for the determination of 2,4-dihydroxy-6-methoxy-3-methylacetophenone and jolkinolide B in Euphorbiae Ebracteolatae Radix. Methods: A Lichrospher C₁₈(4.6 mm ×250 mm,5 μm) column was adopted.The mobile phase consisted of acetonitrile and water with gradient elution(0-5 min,40%A;5-10 min,40%A→50%A;10-30 min,50%A→55%A;30-40 min,55%A→60%A;40-45 min,60%A→70%A),at the flow rate of 1.0 mL·min^-1. The detection wavelength was set at 226 nm and column temperature was 30 ℃. Results: The linear range of 2,4-dihydroxy-6-methoxy-3-methylacetophenone was 0.005-0.863 μg(r=0.9999),the mean recovery was 96.4%(RSD=1.4%,n=6);the linear range of jolkinolide B was 0.003-0.496 μg (r=0.9999),the mean recovery was 96.0%(RSD=2.1%,n=6). Conclusion: The method is specific,accurate and convenient,and can be used for the quality contro1 of Euphorbiae Ebracteolatae Radix.