HPLC法测定尼群地平片的含量

目的: 建立HPLC方法测定尼群地平片的含量。 方法: 采用Agilent C₁₈(150 mm×4.6 mm,5 μm)色谱柱,以乙腈-四氢呋喃-水(20∶ 24∶ 56)为流动相;流速为1.0 mL·min^﹣1;检测波长为237 nm;柱温30 ℃。 结果: 尼群地平在0.02~0.15 mg·mL^-1 (0.4~3.0 μg)的范围内呈良好的线性关系,r＝0.9999(n=6),平均回收率为99.9%,RSD=0.7%。 结论: 建立的HPLC方法简便、准确,与其它方法比较具有明显的优越性。

Objective: To establish an HPLC method for determination of nitrendipine tablets. Methods: Agilent C₁₈(150 mm×4.6 mm,5 μm)column,acetonitrile-tetrahydrofuran-water(20∶ 24∶ 56)as the mobile phase,The flow rate was 1.0 mL·min^-1,The detection wavelength was 237 nm and the column temperature at 30 ℃. Results: The linear range of nitrendipine was obtained between 0.02-0.15 mg·mL^-1(0.4-3.0 μg),r＝0.9999(n=6),the average recovery was 99.9%,RSD=0.7%. Conclusion: The established HPLC method is simple,accurate comparison with other methods have obvious advantages.