RP-HPLC法测定Beagle犬血浆中四环素

目的：建立测定Beagle犬血浆中四环素的RP-HPLC分析方法。方法：Beagle犬血浆样品经001 mol·L^-1草酸酸化、乙腈沉淀蛋白处理后，采用Diamonsil C₁₈柱（200 mm × 4.6 mm,5 μm）分离，流动相为乙腈-0.01 mol·L^-1草酸溶液（15∶85），流速为0.8 mL·min^-1，检测波长为350 nm，内标为土霉素。结果：测定血浆中四环素的线性范围为0.2 ~ 10 μg·mL^-1；日内与日间精密度均小于14.5%，准确度（RE）在 ±5.3 %以内。结论：本方法简便、灵敏、准确，适用于Beagle犬血浆中四环素的分析测定。

Objective:To develop an RP-HPLC method for the determination of tetracycline in Beagle dog plasma.Methods：After acidification with oxalic acid，plasma samples were precipitated with acetonitrile.Chromatographic separation was carried out on a Diamonsil C₁₈ column（200 mm × 4.6 mm,5 μm）using a mobile phase of acetonitrile-0.01 mol·L^-1oxalic acid(15∶85).The flow rate was 0.8 mL·min^-1 and the detection wavelength was 350 nm.Oxytetracycline was used as the internal standard.Results：The calibration curve for tetracycline was linear over the concentration range of 0.2-10 μg·mL^-1.The intra- and inter-day precision was less than 14.5% and the accuracy was within ± 5.3%.Conclusion：The method is simple,sensitive,accurate and suitable for the preclinical pharmacokinetic studies of tetracycline in dog plasma.