HPLC波长切换技术同时测定萸连片中7种成分含量

目的: 建立反相高效液相色谱法同时测定萸连片中盐酸小檗碱、巴马汀、药根碱、吴茱萸碱、吴茱萸次碱、木香烃内酯、去氢木香内酯7种成分含量。 方法: 采用Aglient Extend C₁₈色谱柱(4.6 mm×250 mm,5 μm),流动相为乙腈(A)-0.3%磷酸三乙胺(B),梯度洗脱(0~10 min,70%A→65%A;10~20 min 65%A→50%A;20~40 min,50%A),流速1.0 mL·min^-1;检测波长(0~25 min,265 nm;25~40 min,225 nm);柱温30 ℃。 结果: 盐酸小檗碱、巴马汀、药根碱、吴茱萸碱、吴茱萸次碱、木香烃内酯、去氢木香内酯进样量分别在44.80~112.0 ng(r=0.9994),65.31~163 ng(r=0.9994)57.60~144.0 ng(r=0.9991),59.64~149.1 ng(r=0.9993),58.18~145.5 ng(r=0.9996),14.28~35.69 ng(r=0.9998),14.08~35.20 ng(r=0.9998)的范围内与峰面积呈良好的线性关系;平均回收率(n=5)分别为100.2%(0.72%),100.0%(0.37%),99.7%(0.77%),99.4%(1.9%),100.3%(0.62%),100.8%(1.4%),100.2(0.74%)。 结论: 本法简便、准确,可用于萸连片的质量控制。

Objective: To develop an HPLC method for simultaneous determination of berberine,palmatine base,jatrorrhizine,evodiamine,rutaecarpine,costunolide,hydrocarbon lactone dehydrogenase in evodia and coptis tablets. Method: HPLC was performed on an Agilent Extend C₁₈ analytical column(4.6 mm×250 mm,5 μm) with gradient elution (0-10 min,70% A→65% A;10-20 min,65%A→50%A;20-40 min,50% A)of 0.3% triethylamine phosphate(A)-acetonitrile(B)at the flow rate of 1.0 mL·min^-1;Detection wavelength was 216 nm,and the column teperatuer was maintained at 30 ℃. Results: Berberine,palmatine base,jatrorrhizine,evodiamine,rutaecarpine,costunolide,lactone dehydrogenase had good linearity in the ranges of 44.80-112.0 ng(r=0.9994),65.31-163 ng(r=0.9994),57.60-144.0 ng(r=0.9991),59.64-149.1 ng(r=0.9993),58.18-145.5 ng(r=0.9996),14.28-35.69 ng(r=0.9998),14.08-35.20 ng(r=0.9998),respectively.The average recoveries were 100.2%(0.72%),100.0%(0.37%),99.7%(0.77%),99.4%(1.9%),100.3%(0.62%),100.8%(1.4%),100.2(0.74%). Conclusion: The developed method is rapid,accurate with high repeatability and stability,which is helpful to control the quality of evodia and coptis tablets.