一种改进的大脑皮层神经元原代培养方法的研究

目的: 建立1种简单、高效的新生乳鼠大脑皮层神经元原代培养的方法。 方法: 出生12 h以内的SD乳鼠为研究对象,采用胰酶消化和机械分离法相结合制备细胞悬液;在细胞培养过程中,不加入阿糖胞苷抑制神经胶质细胞生长,而是用2%B27 Neurobasal A medium维持培养。 结果: 神经元生长良好,形成神经元网络系统;通过神经元免疫组织化学鉴定神经元纯度达90%以上。 结论: 此方法是获得纯度较高的神经元的1种可靠方法。

Objective: To establish an simple and effective primary culture skill suitable to neurons of newborn rat cortical tissure in vitro. Method: Objects of study were newborn SD rats which born within 12 h,combination trypsin digestion and mechanical dissociation were adopted to conduct culture;in the course of culture,arabinosid cytarabine(Arc-C) was not put to use to inhibit the outgrowth of glia cell, 2%B27 Neurobasal A medium maintain cell growth. Result: The neurons grew well and formed nerver net system;purity of cultured neurons exceeds 85% by immunohistochemical identification. Conclusion: The technique is a reliable way to obtain higher purity neurons.