HPLC法同时测定清火栀麦片中3种有效成分的含量

目的: 建立清火栀麦片中栀子苷和穿心莲内酯、脱水穿心莲内酯的HPLC测定方法。 方法: 采用CLC-ODS C₁₈(4.6 mm×150 mm,5 μm)色谱柱;流动相为甲醇-乙腈-水,梯度洗脱,流速1.0 mL·min^-1;检测波长:238 nm(0~10 min,栀子苷),224 nm(10.1~25 min,穿心莲内酯),250 nm(25.1~30 min,脱水穿心莲内酯)。 结果: 栀子苷、穿心莲内酯、脱水穿心莲内酯进样量分别在0.20~1.22 μg、0.06~0.35 μg、0.17~1.03 μg范围内有良好的线性关系(r=0.9999)。平均回收率(n=6)分别为98.2%,97.8%,97.9%;RSD分别为0.74%,1.1%,1.0%。 结论: 该方法简便、快捷、准确,可用于该制剂的质量控制。

Objective: To establish an HPLC method for the determination of geniposide,andrographolide and dehydroandrographolide in Qinghuo Zhimai tablets. Methods: CLC-ODS C₁₈ (4.6 mm×150 mm,5 μm) column was adopted;The mobile phase consisted of methanol-acetonitrile-water with gradient elution at the flow rate of 1.0 mL·min^-1;The detection wavelength was 238 nm(0-10 min) for geniposide,224 nm(10.1-25 min) for andrographolide,and 250 nm(25.1-30 min) for dehydroandrographolide. Results: The linear ranges of geniposide,andrographolide and was dehydroandrographolide were 0.20-1.22 μg,0.06-0.35 μg,and 0.17-1.03 μg,respectively.The average recoveries(n=6) of geniposide,andrographolide and was dehydroandrographolide were 98.2%(RSD=0.74%),97.8%(RSD=1.1%),and 97.9%(RSD=1.0%),respectively. Conclusion: The method is simple,rapid,and can be used for the determination of three effective components in Qinghuo Zhimai tablets.