运用GC-MS结合PCA技术对川白芷与杭白芷挥发油成分的比较分析

目的: 运用GC-MS结合主成分分析(PCA)技术对川白芷与杭白芷挥发油成分进行比较分析。 方法: 采用水蒸气蒸馏法提取川白芷和杭白芷挥发油,运用气相色谱-质谱联用技术,结合计算机检索对其挥发油进行分离鉴定,并结合PCA技术对结果进行验证。 结果: 共鉴定出川白芷和杭白芷中122个挥发油成分,其中有57个共有成分,两者所含的挥发油在组分和含量上存在一定的差异,PCA分析亦进一步验证了其异同。 结论: 川白芷与杭白芷挥发油含有大量共有成分,但在组分和含量上存在一定的差异,本研究为川白芷与杭白芷药材的鉴别及质量控制提供了重要依据。

Objective: To analyze the chemical components of the essential oil from two varieties of Radix Angelicae Dahuricae by GC-MS combined with principal components analysis(PCA). Methods: The essential oil was extracted from Radix Angelicae Dahuricae by steam distillation;the chemical components of the essential oi1 were detected by GC-MS;Peaks and their corresponding compounds were identified through database index.The results were validated by PCA. Results: 122 chief components from two varieties of Radix Angelicae Dahuricae were identified,57 components were noted in both.There were some differences between the two kinds.PCA was further used in validating the differences and similarities. Conclusion: The two kinds of Radix Angelicae Dahuricae contained plenty of common components, but there are still some differences in composition and content between them.This study serves as a scientific basis for the further identification and quality control of the two different species of Radix Angelicae Dahuricae.