HPLC-DAD-ELSD法同时测定酸枣仁中斯皮诺素、酸枣仁皂苷A和B的含量

目的: 建立同时测定酸枣仁中斯皮诺素、酸枣仁皂苷A和B含量的HPLC-DAD-ELSD方法。 方法: 采用Grace Brava C₁₈-BDS(250 mm×4.6 mm,5 μm)色谱柱,以乙腈(A)-水(B)为流动相进行梯度洗脱,流速1.0 mL·min^-1,二极管 阵列检测器的检测波长为335 nm,蒸发光散射检测器的漂移管温度为100 ℃,载气流速为2.9 L·min^-1,柱温为25 ℃,。 结果: 斯皮诺素、酸枣仁皂苷A和B的线性范围分别为59.40~594.0 μg·mL^-1(r=0.9996),49.50~900.0 μg·mL^-1(r=0.9998),20.36~407.2 μg·mL^-1(r=0.9999);平均加样回收率(n=6)分别为96.9%(RSD=1.8%),101.2%(RSD=2.5%),100.3%(RSD=2.9%)。 结论: 该方法准确、可靠,在同一色谱条件下实现多指标成分的同时测定,为酸枣仁的全面质量评价提供参考。

Objective: To establish an HPLC-DAD-ELSD method for the simultaneous determination of spinosin,jujuboside A and jujuboside B in Semen Ziziphi Spinosae. Methods: The assay was performed on a Grace Brava C₁₈-BDS(250 mm×4.6 mm,5 μm) column by a gradient elution program with acetonitrile-water as mobile phase at a flow rate of 1.0 mL·min^-1.The detection wavelength of DAD was
[335] nm and the evaporator tube temperature of ELSD was set at
[100] ℃ with the nebulizing gas flow-rate of 2.9 L·min^-1.The temperature of column was kept at
[25] ℃. Results: The linear ranges of spinosin, jujuboside A and jujuboside B were 59.40-594.0 μg·mL^-1(r=0.9996),49.50-900.0 μg·mL^-1(r=0.9998),20.40-407.2 μg·mL^-1(r=0.9999),respectively.The average recoveries(n=6) were 96.9%(RSD=1.8%),101.2%(RSD=2.5%),100.3%(RSD=2.9%),respectively. Conclusion: The method is accurate and reliable for simultaneous determination of multiple index components under the same chromatographic condition, and provides a reference for comprehensive quality evaluation of Semen Ziziphi Spinosae.