RP-HPLC法测定京大戟中大戟二烯醇的含量

目的: 对京大戟中大戟二烯醇进行含量测定。 方法: 采用反相高效液相色谱法,使用Agilent ZORBAX Eclipse XDB-C₈(150 mm×4.6 mm,5 μm)色谱柱,流动相为乙腈-水(92∶ 8),流速1.0 mL·min^-1,检测波长210 nm,柱温25 ℃。 结果: 大戟二烯醇进样量在1.388~10.41 μg范围内有良好的线性关系(r=0.9999),平均回收率(n=6)为97.1%(RSD=1.7%)。 结论: 本方法能够准确测定京大戟中大戟二烯醇的含量。

Objective: To develop an HPLC method for the determination of euphol content in Radix Euphorbiae Pekinensis. Methods: The chromatographic procedure was carried out on a ZORBAX Eclipse XDB-C₈(150 mm×4.6 mm,5 μm) column.The mobile phase was acetonitrile-water(92∶ 8) at a flow of 1.0 mL·min^-1 and the detection wavelength was 210 nm.The temperature of column was 25 ℃. Results: The linear range of euphol was 1.388-10.41 μg(r=0.9999),and the average recovery(n=6) was 97.1% with RSD of 1.7%. Conclusion: This method is accurate,reliable and sensitive for determination of euphol in Radix Euphorbiae Pekinensis.