中药槐花饮片RP-HPLC特征图谱研究

目的: 采用RP-HPLC方法对槐花、炒槐花和槐花炭的特征图谱进行研究,探讨炮制对槐花化学成分的影响。 方法: 采用大孔吸附树脂法对槐花、炒槐花和槐花炭3种样品进行前处理,分别得到各自的20%乙醇、50%乙醇和95%乙醇洗脱部位。以RP-HPLC法对这些部位分别进行特征图谱分析。色谱柱为Kromasil C₁₈(4.6 mm×250 mm,5 μm)柱,两相梯度洗脱,其中20%和50%部位的有机相为含0.4%冰醋酸的甲醇-乙腈-四氢呋喃系统(5∶ 1∶ 0.1),95%部位的有机相为0.4%冰醋酸的甲醇;水相均为含0.4%冰醋酸的水,流速均为0.8 mL·min^-1,检测波长320 nm。以"中药色谱指纹图谱相似度评价系统(2004 A版)"对实验数据进行相似度分析。 结果: 槐花、炒槐花和槐花炭3个洗脱部位的共有峰数目依次为12,12,18;10,10,8;18,18,12。槐花和炒槐花特征图谱相似度均高于0.9,而槐花和槐花炭的特征图谱相似度均低于0.6。 结论: 炒槐花的成分组成与槐花相同,仅在相对含量上稍有差异,而槐花炭的成分组成和含量均变化较大。

Objective: To explore the effect on constituents of Sophorae Flos by processing,according to the specific chromatogram of Sophorae Flos,Sophorae Flos Tostus and Flos Sophorae Carbonisatum by HPLC. Methods: Resin was used in the preparation of samples,and the residue of the three pieces of Sophorae Flos was detected by HPLC.The column was a reverse-phase column(Kromasil C₁₈,5 μm,4.6 mm×250 mm).Separation was performed by linear gradient elution using organic phase and water phase.The organic phase for 20% and 50% ethanol eluates was a mixture of methanol,acetonitrile and (5∶ 1∶ 0.1) containing glacial acetic acid(0.4%),and for 95% ethanol eluates was methanol containing acetic acid(0.4%).The water phase was water containing glacial acetic acid(0.4%).The flow rate was 0.8 mL·min^-1.The column temperature was set at 320 nm.Data analysis was performed by a professional software named Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2004 A). Results: The common peaks of specific chromatogram in the three ethanol eluates of Sophorae Flos,Sophorae Flos Tostus and Sophorae Flos Carbonisatum were 12,12,18;10,10,8;18,18,12;separately.The similarities was over 0.9 between Sophorae Flos,Sophorae Flos Tostus and was below 0.6 between Sophorae Flos and Sophorae Flos Carbonisatum. Conclusion: The series and contents of constituents in the Sophorae Flos Tostus were same as Sophorae Flos.After carbonized,the content and composition of the constituents of Sophorae Flos changed significantly.