HPLC法同时测定独活葛根汤浸膏中甘草酸和蛇床子素的含量

目的: 建立同时测定独活葛根汤浸膏中甘草酸和蛇床子素的含量测定方法。 方法: 采用反相高效液相色谱法,色谱柱为ZORBAX Eclipse XDB-C₁₈(4.6 mm×250 mm,5 μm),流动相为甲醇-0.2 mol·L^-1醋酸铵-冰醋酸(67∶ 33∶ 1),检测波长为254 nm,流速为1.0 mL·min^-1。 结果: 甘草酸的线性范围为0.01~0.26 mg·mL^-1,r=0.9999,平均回收率为99.2%,RSD为0.9%(n=6);蛇床子素线性范围为0.01~0.26 mg·mL^-1,r=0.9999,平均回收率为98.8%,RSD为0.5%(n=6)。 结论: 该方法快捷、准确、简便,重现性好,可用于独活葛根汤浸膏的质量控制。

Objective: To establish an HPLC method for simultaneous determination glycyrrhizic acid and osthole in"Extract of Duhuo Gegen Decoction". Methods: The chromatographic conditions include ZORBAX Eclipse XDB-C₁₈(4.6mm×250 mm,5 μm) column ,methanol-0.2 mol·L^-1 ammonium acetate-acetic acid(67∶ 33∶ 1)as mobile phase ;The flow rate was 1.0 mL·min^-1 and monitored at 254 nm. Results: The method had good linear relationship within the range of 0.01–0.26 mg·mL^-1 for glycyrrhizic acid(r=0.9999),0.01–0.26 mg·mL^-1 for osthole(r=0.9999);The average recovery of glycyrrhizic acid was 99.2%(RSD=0.9%),of ostenole was 98.8%(RSD=0.5%). Conclusion: The method is rapid,accurate,simple and with good reproducibility.It can be used for the quality control of"Extract of Duhuo Gegen Decoction".