大孔吸附树脂纯化江南卷柏总黄酮的工艺研究

目的: 确定纯化江南卷柏总黄酮的大孔树脂及其工艺参数。 方法: 以总黄酮的含量为指标,从3种不同型号的大孔树脂中筛选纯化江南卷柏总黄酮的最佳树脂;以江南卷柏总黄酮的回收率为指标,通过单因素试验确定该树脂分离纯化的工艺条件。 结果: D101型树脂对江南卷柏总黄酮有较好的吸附和解吸附性能;其纯化江南卷柏总黄酮的较佳工艺条件为:药材量与树脂量的比例为1∶ 3,以8 BV(8倍体积)70%乙醇洗脱,流速为2 BV·h^-1,树脂每次可重复使用3次。在所确定的工艺条件下,江南卷柏总黄酮纯度可达50%以上,回收率可达85%以上。 结论: 该工艺条件可用于江南卷柏总黄酮的纯化。

Objective: To determine the optimum macroporous resin & the parameters of technology purificating total flavonoids from Selaginella moellendorfii Hieron with the resin. Methods: With the content of total flavonoids from Selaginella moellendorfii Hieron as index,screen out the optimal resin which can purify the from 3 different resins,and determin the technology condition purifying the by single-factor experiments. Results: The total flavonoids from Selaginella moellendorfii on D101 macroporous resin showed the best effection of adsorption & desorption.The preferable technology of purifying the total flavonoids was:the ratio of weight of the herbs and resin was 1∶ 3,eluted at a velocity of 2 BV·h^-1 by a volume of 8 BV·h^-1 of 70% alcohol.The resin could be reused for 3 times before treating.Under the technology the recovery rate of total flavonoids was more than 85% and the purity was more than 50%. Conclusion: The D101 macroporous resin is suitable to purify the total flavonoids from Selaginella moellendorfii Hieron.