近红外技术对苦参提取物的定量分析研究

目的: 采用近红外漫反射光谱技术定量分析苦参提取物中苦参碱和氧化苦参碱的总量,指导苦参提取物的制备工艺研究。 方法: 通过实验室自制的样品和市场上购买的样品,在OPUS软件环境下,采用偏最小二乘法建立定量模型,用新的样品作为验证集进行验证。 结果: 模型预测的结果和实验室测定的结果基本一致。 结论: 建立的模型可以较为准确地预测未知苦参提取物中苦参碱和氧化苦参碱的总量,能方便的指导苦参提取工艺研究。

Objective: Near-infrared diffuse reflectance spectroscopy was used for quantitative analysis of sophora flavescens extract to determine the total amount of matrine and oxymatrine and to guide the technology study on preparation of sophora flavescens extracts. Methods: The laboratory-made samples and samples purchased on the market,in OPUS software environment,were used to establish a quantitative models with partial least squares method,and new samples were used as validation to set for validation. Results: The results of model predictions and laboratory measurement results are basically the same. Conclusion: The established model can provide a more accurate forecast of the unknown sophora flavescens extract about the total amount of matrine and oxymatrine,easily guide sophora extraction process.