期刊名称:药物分析杂志 主管单位:中国科学技术协会 主办单位:中国药学会承办:中国食品药品检定研究院 主编:金少鸿 地址:北京天坛西里2号 邮政编码:100050 电话:010-67012819,67058427 电子邮箱:ywfx@nifdc.org.cn 国际标准刊号:ISSN 0254-1793 国内统一刊号:CN 11-2224/R 邮发代号:2-237
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RP-HPLC法同时测定火麻仁油中3种大麻酚类成分的含量
RP-HPLC simultaneous determination of three canabinoids in hemp seed oil
分类号:
出版年·卷·期(页码):2010,30 (9):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------
目的: 建立RP-HPLC法同时测定火麻仁油中大麻二酚、大麻酚和Δ9-四氢大麻酚的含量。 方法: 采用Kromasil C18(4.6 mm×250 mm,5 μm)色谱柱,流动相为乙腈-0.1%醋酸水溶液(73∶ 27),流速1.0 mL·min-1,检测波长220 nm, 柱温35 ℃。 结果: 大麻二酚、大麻酚和Δ9-四氢大麻酚浓度分别在0.125~1.25 μg·mL-1(r=0.9996),0.735~7.35 μg·mL-1(r=0.9997),3.75~37.5 μg·mL-1(r=0.9997)范围内与峰面积呈良好线性关系;平均回收率(n=9)分别为97.2%,98.0%,96.1%。 结论: 本法准确、快速,灵敏度高,可用于火麻仁油质量控制。
-----英文摘要:---------------------------------------------------------------------------------------
Objective: To establish an RP-HPLC method for the determination of cannabidiol,cannabinol and Δ9-tetrehydrocannabinol in hemp seed oil. Methods: A Kromasil C18(4.6 mm×250 mm,5 μm)column with the mobile phase consisting of acetonitrile- 0.1% acetic acid solution(73∶ 27)was adopted.The flow rate was 1.0 mL·min-1,the UV detection wavelength was set at 220 nm and the column temperature was 35 ℃. Results: The calibration curve of cannabidiol,cannabinol and Δ9-tetrehydrocannabinol was linear in the ranges of 0.125-1.25 μg·mL-1(r=0.9996),0.735-7.35 μg·mL-1(r=0.9997)and 3.75-37.5 μg·mL-1(r=0.9997), respectively.The average recoveries(n=9)of cannabidiol,cannabinol and Δ9-tetrehydrocannabinol were 97.2%,98.0% and 96.1%,respectively. Conclusion: The method is sensitive,accurate,rapid and is suitable for the quality control of hemp seed oil.
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