离子色谱法测定注射用头孢他啶中碳酸钠的含量

目的: 建立离子色谱法测定注射用头孢他啶中碳酸钠的含量。 方法 :色谱柱为Ionpac CG12A,CS12A 阳离子交换柱,淋洗液为18 mmoL· L^-1甲烷磺酸,CSRS-ULTRA 4 mm自动抑制循环模式,抑制电流为60 mA,电导检测池温度为35 ℃;进样量为25 μL。由钠离子标准工作曲线直接测得注射用头孢他啶中钠离子的浓度。 结果 :钠离子浓度在2~20 mg·L^-1范围内呈良好的线性关系(r＝0.9992);平均回收率为 100.6％ (n＝9)。 结论 :本方法简单、快速,可以用于注射用头孢他啶中碳酸钠含量的测定。

Objective: To establish an ion chromatography method for the determination of sodium carbonate in ceftazidime for injection. Method: The determination was performed on an Ionpac CS12A column(4 mm×250 mm)with an Ionpac CG12A guard column(4 mm×50 mm) .The mobile phase was a mixture of 18 mmoL· L^-1 methylsulfonic acid.The flow rate was 1.0 mL·min^-1.A conductivity detector and an CSRS suppressor were used. Results: The standard curve was linear over the range of 2-20 mg·L^-1(r＝0.9992).The average recovery was 100.6％(n＝9). Conclusion: The method is simple, accurate, and suitable for the determination of sodium carbonate in ceftazidime for injection.