离子对高效液相色谱法检测中成药及保健食品中添加的化学降糖药
Ion-pair HPLC determation of anti-diabetic agents in traditional Chinese medicines and health care products
分类号：
出版年·卷·期（页码）：2010,30 (6):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
<p>目的: 建立离子对高效液相色谱法检测中成药及保健食品中添加二甲双胍、苯乙双胍、格列齐特、格列美脲、格列本脲、格列吡嗪、甲苯磺丁脲、罗格列酮、吡格列酮共9种化学降糖药。 方法: 采用Agilent ZORBAX-Eclipse XDB-18(4.6 mm×250 mm,5 μm)色谱柱分离,流动相为0.02 mol·L<sup>-1</sup>磷酸二氢钾溶液(含2 mmol·L<sup>-1</sup>十二烷基硫酸钠)-乙腈(62.5∶ 37.5)(用1 mol·L<sup>-1</sup>氢氧化钠溶液调节pH至6.5),流速1.0 mL·min<sup>-1</sup>,检测波长230 nm,柱温30 ℃。 结果: 9种化学降糖药能完全分离;具有宽的线性范围和良好的线性关系(<em>r</em>>0.9992),检出限为5.9~43.5 μg·L<sup>-1</sup>。 结论: 该方法用于中药及保健食品中化学降糖药的检测具有快速、简便、高效的特点。</p>
-----英文摘要：---------------------------------------------------------------------------------------
<p>Objective: To develop an ion-pair HPLC method for the simultaneous estimation of nine anti-diabetic agent——metformin,phenformin,gliclazide,glimepiride,glibenclamide,glipizide,tolbutamide,rosiglitazone,pioglitazone,which is in traditional Chinese medicines and health care products. Method: An Agilent ZORBAX-Eclipse XDB-18(4.6 mm×250 mm,5 μm)column was adopted with a mobile phase composed of 0.02 mol·L<sup>-1</sup>KH<sub>2</sub>PO<sub>4</sub>(containing 2 mmol·L<sup>-1</sup> SDS)-acetonitrile (62.5∶ 37.5,adjusted to pH 6.5 using 1 mol·L<sup>-1</sup> NaOH solution) at a flow rate of 1.0 mL·min<sup>-1</sup>.Detection was carried out at 230 nm,and the column temperature was 30 ℃. Results: The 9 type of chemical anti-diabetic agents were separated well under the selected chromatographic conditions.The linear regression equation for each component was obtained,and the correlation coefficient (<em>r)</em>were better than 0.9992.The limits of detection were 5.9-43.5 μg·L<sup>-1</sup>. Conclusion: The method can be used for the detection of the anti-diabetic agents in traditional Chinese medicines and health care products,and it is rapid,convenient,economic and specific.</p>
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