RP-HPLC法测定千里光药材中金丝桃苷的含量___

目的: 建立千里光药材中金丝桃苷的RP-HPLC含量测定方法。 方法: 采用Shiseido Capcell Pak MG Ⅱ C₁₈色谱柱(4.6 mm×250 mm,5 μm),流动相为乙腈-0.2%醋酸(18∶ 82),流速1.0 mL·min^-1,检测波长360 nm,柱温35 ℃。 结果: 金丝桃苷进样浓度在0.9980~199.6 μg·mL^-1范围内呈现良好线性关系(r=0.9994,n=6);25%,50%,75%,100%,150%浓度水平下加样回收率在99.7%~103.5%之间,RSD<2.0%;重复性及精密度良好,且具有较好的色谱条件耐用性。 结论: 所建立HPLC方法灵敏、准确,重现性好,适用于千里光药材中金丝桃苷的含量测定,可用于千里光药材的质量控制,为千里光药材2010年版中国药典的质量标准研究奠定基础。

Objective: To develop an RP-HPLC method for quantitation of hyperoside in Herba Senecionis Scandentis. Methods: Chromatography was performed on a Shiseido Capcell Pak MG Ⅱ C₁₈ (4.6 mm×250 mm, 5 μm) column at 35 ℃ using an isocratic elution of acetonitrile-0.2% acetic acid in water(18∶ 82) at the flow rate of 1.0 mL·min^-1. And detection wavelength was 360 nm. Results: Good linearity was obtained over the range of 0.9980-199.6 μg·mL^-1(r=0.9994, n=6). And recoveries at different concentration levels were between 99.7% and 103.5% with RSDs less than 2.0%. Good reproducibility and precision as well as ruggedness were also achieved. Conclusion : The new developed RP-HPLC method is sensitive, accurate and reliable enough for the quantitation of hyperoside in Herba Senecionis Scandentis thus can be used for the quality evaluation on Herba Senecionis Scandentis.

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