Objective: To evaluate the best experimental system of the seven kinds of plasma for the assay of heparin. Methods: Coagulometer was used in the assay of activated partial thromboplastin time (APTT) as the British Pharmacopoeia (BP2008). Different plasma used in biological assay of heparin was evaluated by clotting time and the average recovery rate of heparin standard. Results: The reliability tests of the assays on fresh rabbit plasma, fresh sheep plasma, frozen sheep plasma, fresh pig plasma, frozen pig plasma, fresh rat plasma and frozen human plasma were all passed. The linear regression was significant (P<0.01). The parallel deviation, the conic curve and the reverse conic curve were not significant (P>0.05). The average recovery rates (%, n=3) of heparin on the seven kinds of plasma were 99.41±1.02, 100.47±3.48, 100.71±0.89, 100.94±0.71, 102.40±0.22, 100.84±1.06 and 101.55±3.16. The average FL% (%, n=3) were 2.63±0.21, 5.03±1.33, 4.90±0.37, 10.55±1.32, 4.04±0.79, 10.69±4.18, 7.81±0.35. Conclusion: Both the assays of heparin on fresh rabbit plasma and frozen sheep plasma are stable, accurate and precise. Fresh rabbit plasma and frozen sheep plasma are much better to be the experimental system of the biological assay of heparin than others.
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