HPLC法测定苦芪滴丸中苦参的5个生物碱含量___

 

 

 

目的：采用高效液相色谱法对苦芪滴丸中苦参的5个单体生物碱(槐果碱、苦参碱、氧化槐果碱、槐定碱、氧化苦参碱)进行定量分析。方法：采用Inertsil NH2(150 mm×4.6 mm,5 μm)色谱柱，以乙腈(A)-无水乙醇(B)-3%磷酸水溶液(C)为流动相，梯度洗脱，流速1.0 mL·min-¹，检测波长210 nm。结果：苦参的5个单体生物碱含量稳定。槐果碱、苦参碱、氧化槐果碱、槐定碱、氧化苦参碱进样量分别在0.14~0.49，0.38~1.30，0.06~0.20，0.05~0.18，0.13~0.44 μg范围内呈良好的线性关系；r分别为0.9998，0.9997，0.9991，0.9991，0.9991。平均回收率(n=5)分别为98.2%，98.3%，97.7%，98.3%，98.9%。结论：方法可靠、实用，可用于苦芪滴丸的质量控制。

 

 

Objective:To develop an HPLC method for determination of 5 alkaloids of Radix Sophorae Flavescentis in Kuqi dropping pills.Methods:Inertsil NH2(150 mm×4.6 mm,5 μm) was adopted;The mobile phase was acetonitrile(A)-absolute alcohol(B)-3% phosphonic acid water solution(C) with gradient elution at a flow rate of 1.0 mL·min-1,and the detection wavelength was 210 nm.Results:The content of 5 alkaloids of Radix Sophorae Flavescentis was stable.The method has a good linearity in the ranges of 0.14-0.49 μg(r=0.9998) for sophocarpine,038-1.30 μg (r=0.9997) for matrine,0.06-0.20 μg(r=0.9991) for oxysophocarpine,0.05-0.18 μg(r=0.9991) for sophoridine,and 0.13-0.44 μg(r=0.9991) for oxymatrine;The average recoveris were 98.2%,98.3%,97.7%,98.3% and 98.9%,respectively.Conclusion:This method is dependable,simple and practica1,and may be used to control quality of Kuqi dropping pills.

1ChP(中国药典)2005.Vol Ⅰ(一部)：141

2JIN Li-xia(金莉霞),CUI Yan-yan(崔燕岩),ZHANG Guan-de(张观德).Sophora alkaloids by HPLC(苦参生物碱高效液相色谱法测定).Acta Pharm Sin(药学学报),1993,8(2):136

3MA Chang-hua(马长华),CAO Tian-hai(曹天海).Determination of alkaloids in Sophora tonkinensis Gapnep. by HPLC(HPLC法测定山豆根中苦参碱和氧化苦参碱的含量).Chin J Pharm Anal(药物分析杂志),2000,20(6):408

4LI Pei-fan(李培凡),WANG Zhi(王智),SHEN Bin(沈彬),et al.RP-HPLC simultaneous determination of matrine,oxymatrine,sophocarpine and sophoramine content(反相高效液相色谱同时测定苦参碱、氧化苦参碱、槐果碱和槐胺碱的含量).Lishizhen Med Mater Med Res(时珍国医国药),2004，15(2):76

5CHEN Shu-xia(陈曙霞).Pharmacological research of Sophora alkaloids(苦参生物碱的药理研究进展).Chin Tradit Pat Med(中成药),2003,25(1):75

6FAN Xin-rong(范欣戎),TIAN Song-jiu(田颂九),ZHANG Zheng-xing(张正行),et al.HPLC identification and determination of some alkaloids in Sophora flavescens Ait.(HPLC法鉴别测定苦参药材中多种生物碱成分).Chin J Pharm Anal(药物分析杂志),2006,26(3):329

7ZHANG Shu-yun(张淑运),WANG Ye(王烨),HE Xi-rong(何希荣),et al.Determination of alkaloids in Sophora flavescens Ait.(苦参药材中生物碱的含量测定).Chin J Exp Tradit Med Form(中国实验方剂学志),2005,11(6):3

8LIU Rui-ming(刘瑞明),LI Song(李松).Study on quality standard of Moji granules(磨积颗粒质量标准的研究).Res Pract Chin Med(现代中药研究与实践),2007,21(1):46