荧光光谱法研究抗癌药物羟基喜树碱与牛血清白蛋白的相互作用___
Studies on the interaction between anticancer drug hydroxycamptothecin and bovine serum albumin by fluorescence spectroscopy
分类号：
出版年·卷·期（页码）：2010,30 (1):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
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Objective:To study the interaction of bovine serum albumin(BSA) with anticancer drug hydroxycamptothecin(HCPT) by fluorescence quenching spectra and synchronous fluorescence spectra.Methods:The Stern-Volmer curve and double logarithm equation and Förster theory of non-radiation energy transfer was used to deal with the dates of fluorescence quench,and the synchronous spectrum was used to investigate the conformational changes of BSA.Results: The binding distances(r) of hydroxycamptothecin to BSA was 3.08 nm(292 K);the binding constants(Kb) were 1.260×106 L·mol-1(292 K) and 3.881×104 L·mol-1(311 K),respectively.The thermodynamic parameters △rHm>0,△rSm>0 and △rGm<0. Conclusion:The static quenching exits between BSA and anticancer drug hydroxycamptothecin super-molecular complex,and interaction of them is mainly driven by hydrophobic force.
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