HPLC法测定新疆一枝蒿全草中一枝蒿酮酸的含量___
HPLC determination of rupestric acid in Artemisia rupestris L.
分类号：
出版年·卷·期（页码）：2010,30 (1):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
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-----英文摘要：---------------------------------------------------------------------------------------
Objective:To develop an HPLC method for determination of rupestric acid in Artemisia rupestris.Methods:Rupestric acid was separated by DiamonsilTM C18(4.6 mm×250 mm,5 μm) column with methanol-water(2.5% acetic acid)(1∶1) as mobile phases at a flow rate of 1 mL·min-1;The detection wavelength was 244 nm,and the column temperature was 25 ℃.Results:The linear range of rupestric acid was in 0.158-0.948 μg;The average recovery(n=5) was 97.36%,RSD=1.5%.Conclusion:The method is simple,accurate and reliable.
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1 LIU Yong-min(刘勇民),LIU Wei-xin(刘伟新),YI Wu-ti·Yi Ke Mu(伊吾提·依克木),et al.Uygur Yaozhi(维吾尔药志).Urumchi(乌鲁木齐):Xinjiang People’s Publishing House(新疆人民出版社),1985
2 LIU Yong-min(刘勇民),YU De-quan(于德泉).Studies on the chemical constituents of Artemisia rupestris L.(新疆一枝蒿化学成分研究).Acta Pharm Sin(药学学报),1985,20(7):514
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