Objective:To develop an RP-HPLC method for simultaneous determining chlorogenic acid,orientin,vitexin,linarin and luteolin in Yinlian extract.Methods:The separation was carried out on Thermo Hypersil BDS C18(250 mm×4.6 mm,5 μm) column with a mobile phase of 0.4% acetic acid solution(A) and acetonitrile(B) in gradient mode(The initial ratio of 0.4% acetic acid solution was 93% and decreased to 70% within 25 minutes,and the ratio of 0.4% acetic acid solution was maintained 70% within following 10 minutes),at a flow rate of 0.7 mL·min-1.The detection wavelength was set at 330 nm and the analysis was performed at room temperature.Results:The linear ranges of chlorogenic acid,orientin,vitexin,linarin and luteolin were 0.290-11.6 μg·mL-1(r=0.9998),0.350-14.0 μg·mL-1(r=0.9997),0.320-12.8 μg·mL-1(r=0.9997),0280-11.2 μg·mL-1(r=0.9995),0.110-4.40 μg·mL-1(r=0.9999),respectively.The average recoveries(n=6)of chlorogenic acid,orientin,vitexin,linarin and luteolin was 99.4%,106%,102%,106%,92.3% and with RSD of 2.6%,2.1%,2.8%,1.4%,1.5%,respectively.Conclusion:This method is fast,simple,reproducible for the simultaneous determination of chlorogenic acid,orientin,vitexin,linarin and luteolin in Yinlian extract.
