白藜芦醇衍生物在大鼠尿液中代谢产物结构及代谢途径研究___

Objective:To identify the major metabolites and metabolic pathways of resveratrol derivative (E)-3,5,4'-trimethoxystilbene,BTM-0512) in rat urine.Methods:Samples were pretreated by using solid-phase extraction technique (SPE) with C18 cartridges,and a LC/MS/MS method with electrospray ionization (ESI),positive ion mode and collision induced dissociation (CID),was used to elucidate the structures of the major metabolites of BTM-0512UV，IR and NMR were used to identify the structures of M1 Results:The results proved that the LC/MS/MS method established was simple,reliable and sensitive，and can be easily screened and identified the structures of the major metabolites of BTM-0512 in rat urine;it was better to understand its in vivo metabolism.Conclusion:The main metabolites of BTM-0512 are three phase Ⅰand one phase Ⅱmetabolites in rat urine,the possible metabolic pathways is proposed for the first time.The structure of M1 is authenticated by combining with spectra technologies.

 

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