伊文思蓝共振瑞利散射法测定穿琥宁___
RRS method for determining potassium dehydroandrographolide succinate in its injection solution with evans blue
分类号：
出版年·卷·期（页码）：2009,29 (12):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的：通过实验，建立一种测定穿琥宁的新方法。方法：在pH 45的酸性溶液中，取适量穿琥宁溶液和伊文思蓝溶液混合，以二次蒸馏水稀释，放置一段时间后在荧光光度计上以λem=λex方式进行同步扫描。结果：伊文思蓝和穿琥宁能够迅速反应生成离子缔合物，导致共振瑞利散射（RRS）光谱的改变，产生比较特征的新的RRS光谱，最大峰值在717 nm，并且在25~900 mg·L-1范围内，RRS强度和穿琥宁的浓度成正比。结论：我们建立了测定穿琥宁的共振瑞利散射法。该法灵敏度较高，检测限可达到45 ng·mL-1，用于检测某些穿琥宁样品中穿琥宁的含量，结果较满意。
-----英文摘要：---------------------------------------------------------------------------------------
Objiective:We wish to found a method to determine PDS in medicine.Method:In pH 45 HAc-NaAc butter solution,potassium dehydroandrographolide succinate （PDS）,water and evans blue (EB) with proper properation were mixed.After a while,it was put in Hitachi F-2500 and determined the resonance rayleigh scattering(RRS) spectra.Result:EB reacts rapidly with PDS to form complexes,which results in great enhancement of resonance rayleigh scattering（RRS）and a new RRS spectrum.The maximum RRS peak was near 717 nm.RRS intensity was proportional to the concentration of PDS in the range of 25-900 mg·L-1.Conclusion:A method can be used to determine PDS based on our experiments.The method has high sensitivity and the detect limit (3σ) for PDS is 45 ng·mL-1.The method has been used for the determination of total amounts of PDS in samples with satisfactory results.
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