高效液相色谱法测定茅苍术药材中β-桉叶醇与苍术素的含量___

目的：建立茅苍术药材中β-桉叶醇和苍术素含量的测定方法。方法：采用高效液相色谱法，色谱柱为Comatex C18-AB(150 mm×46 mm，5 μm)柱，流动相为乙腈-水-碳酸氢铵(65∶35∶02)，流速10 mL·min-1，检测波长203 nm，柱温为室温(25 ℃)。结果：被测定峰与相邻峰可达到基线分离；β-桉叶醇和苍术素的线性范围分别为23~1150 μg·mL-1(r=1000)和112~560 μg·mL-1(r=09999)；平均回收率(n=9)分别为993%和999%。结论：本方法准确、简便，重复性好，可作为茅苍术中β-桉叶醇和苍术素的含量测定方法。

Objective:To develop an RP-HPLC method for simultaneous determination of β-eudesmol and atractylon in Atractylodes lancea.Methods:The determination was performed by HPLC method,a Comatex C18-AB column(150 mm×46 mm，5 μm) was adopted with a mobile phase of acetonitrile-water-ammonium bicarbonate(65∶35∶02) at a flow rate of 10 mL·min-1;The detection wavelength was set at 203 nm and the column temperature was 25 ℃.Results:The linear ranges of β-eudesmol and atractylon were 23-1150 μg·mL-1(r=1000) and 112-560 μg·mL-1(r=09999),respectively;The average recoveries(n=9) of the two components in Atractylodes lancea were 993% and 999%,respectively.Conclusion:The method is simple,precise and reliable for determination of β-eudesmol and atractylon in Atractylodes lancea (Thunb.) DC..

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