目的：比较不同纳米银医疗产品的体外细胞毒性的差异，为纳米银医疗产品的细胞毒性标准的制定和体外安全性评价提供参考。方法：将不同纳米银医疗产品制备成相应的浸提液，与L929 细胞接触培养，通过倒置相差显微镜观察其形态,采用MTT(四唑盐) 法量化细胞毒性,计算相对增值率(RGR) ,并进行细胞毒性评价。结果：在18种不同的纳米银抗菌医疗产品中，有16.7%的产品其细胞毒性为2级; 有5.6%的产品其细胞毒性为3级；而有77.8%的产品其细胞毒性为4级。结论：不同的纳米银医疗产品的体外细胞毒性有较大差异。
Objective: To compare the cellular toxic response with respect to the different nano-silver products and evaluate the biocompatibility of them. Methods: 18 nano-silver products were comparison with cytotoxicity in vitro. L929 cells were used in this study. The cellular morphology was observed by phase contrast inverted microscopy and proliferation of the cells was examined using mitochondrial function (MTT) assay. Relative growth rate (RGR) was calculated and the grade of cytotoxicity was evaluated. Results: 16.7% of those nano-silver products were evaluated with grade two and 5.6% of them were evaluated with grade three. 77.8% of those nano-silver products were evaluated with grade four. Conclusions: It showed there was different cytotoxicity during those nano-silver products.
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