UPLC-MS/MS测定降糖类中成药及保健品中11种化学药___

目的：建立一种测定降糖类中药制剂及保健品中添加11种化学药的超高效液相-串联四级杆质谱方法。方法：使用超高效液相-串联四极杆质谱的多反应监测（MRM）模式测定。以Waters ACQUITY UPLCTM BEH C18（2.1mm100 mm，1.7 m）色谱柱进行分离，甲醇-10 mmol•L-1醋酸铵溶液梯度洗脱，流速：0.2 mL•min-1。离子化模式：ESI+。结果：11种化学药测定的线性范围在0.5～200.0 g•L-1之间，r分别在0.9976~0.9997之间，检出浓度分别在0.1~3.6 ng•g-1之间，3个水平的回收率分别在90.0 %～118.7 %间。结论：该方法快速、准确、灵敏，可用于降糖类中药制剂及保健品中添加11种化学药的测定。

Objective: To establish a method for determination of 11 anti-diabetic medicines in Chinese traditional patent medicine and health food by UPLC triple-quadrupole tandem mass spectrometer. Method: Using UPLC-MS/MS to determinate by multi-reactions monitoring (MRM) technology. The separation was performed on a Waters ACQUITY UPLCTM BEH C18（2.1mm100 mm，1.7 m）column with a gradient mobile phase of methanol - 10 mmol•L-1 ammonium acetate with a flow rate of 0.2 mL•min-1. Ion mode: ESI+. Results: The standard curves of the 11 chemical medicines showed good linearity over the concentration range of 0.5 -200 g•L-1 and r = 0.9976 - 0.9997， respectively. The concentration of 11 chemical medicines were between 0.1 -3.6 ng•g-1 and the average recoveries were 90.0 %～118.7 % at three levels,. Conclusion: The method is rapid and sensitive, and suitable for the determination of 11 chemical medicines in Chinese traditional patent medicine and health food.