HPLC法同时测定川芎药材中3种有效成分的含量___

Objective： To develop an HPLC method for Ferulic acid, 6,7-di-hydroxyligustilide and 4-hydroxy-3-butylphthalide in Rhizoma Chuanxiong. Methods   A SHINWA -ODS（250 mm × 4.6 mm i.d,5 µm）column was used to separate the sample at the column temperature of 30 ℃. Methanol was mobile phase A and0.1% aetic acid was mobile phase B with gradient elution (0-20 min, A: 30→60, B: 70→40; 20-33 min, A: 60→70, B: 40→30) at a flow rate of 1.0 mL·min^-1 .The detector was DAD. The detection wave lengths of ferulic acid,6,7-di-hydroxyligustilide, 4-hydroxy-3-butylphthalide were at 320 nm, 278 nm and 254 nm. Results The calibration curve was linear over the concentration of 7.15-71.50 ng·mL^-1 for ferulic acid (r = 0. 9999);. 39.90-399.00 ng·mL^-1 for 6,7-di-hydroxyligustilide(r = 0.9997) 0.29-2.88 ng·mL^-1 for 4-hydroxy-3-butylphthalide(r = 0.9998). The average recovery of Ferulic acid; 6,7-di-hydroxyligustilide and 4-hydroxy-3-butylphthalide were 103.2%, 102.3%, 102.2% and the RSD were1.9%, 1.7%, 2.0%, respectively. Conclusion This method is smiple and reproducible, which can be used for the quality control of Rhizoma Chuanxiong.