RP-HPLC法测定咽炎片中哈巴俄苷、肉桂酸、丹皮酚、芸香苷、黄芩苷的含量___

摘要 目的：建立高效液相色谱法同时测定咽炎片中哈巴俄苷、肉桂酸、丹皮酚、芸香苷和黄芩苷5种有效成分的含量。方法：采用Kromacil C18柱(4.6 mm×200 mm，5 µm)，以甲醇-0.05%磷酸水溶液为流动相梯度洗脱，流速1 mL•min-1，于278 nm波长下检测，柱温为35 ℃。结果：哈巴俄苷、肉桂酸、丹皮酚、芸香苷和黄芩苷进样浓度分别在0.200～4.00，0.208～4.16，0.610～12.2，0.960～19.2，1.00～20.0 µg•mL-1范围内线性关系良好；平均回收率(n=6)分别为100%，102%，101%，98.3%，101%。结论：本方法简便，准确可靠，重现性好，能有效测定该药品主要成分的含量，具有较强的实用价值。

Abstract Objective:To develop an HPLC method for determining the content of harpagoside,cinnamic acid,paeonol,rutoside and baicalin in Yanyan tablets.Methods:A Kromacil C18 column(4.6 mm×200 mm,5 µm) was adopted,the mobile phase was methanol-water(contained 0.05% phosphoric acid) for gradient elution with flow rate of 1.0 mL•min-1.The detection was set at 278 nm,and the column temperature was maintained at 35 ℃.Results:Good linearities were obtained from 0.200 to 4.00, 0.208 to 4.16, 0.610 to 12.2, 0.960 to 19.2, 1.00 to 20.0 µg•mL-1 for harpagoside, cinnamic acid, paeonol, rutoside,and baicalin,respectively;The mean recoveries(n=6) of 5 components were 100%,102%, 101%, 98.3%, 101%,respectively.Conclusion:The method is simple and accurate with good reproducibility. It has been successfully applied to the quantitave determination of the main components of Yanyan tablets.