UPLC-MS/MS法测定人血浆中盐酸尼卡地平___

Objective: To establish an UPLC-MS/MS method for the determination of nicardipine hydrochloride in human plasma.  Methods: Nicardipine hydrochloride and internal standard were extracted from plasma with methyl tert-butyl ether and determinated by UPLC-MS/MS using electrospray ionization (ESI^-).  The drugs were detected on multiple reaction monitoring mode by the transitions precursor to the product ion (m/z 480.3®314.8 and m/z 419.2®343.0).  Results: Calibration curve was linear in the range from 0.1-40.0 ng·mL^-1 with a correlation coefficient of 0.9998.  The intra- and inter-day precision (RSD) were all less than 12.1%.  Conclusion: The UPLC-MS/MS method for the quantitative determination of nicardipine hydrochloride in human plasma presented simple process, sensitive detection and acceptable precision.  Therefore, it can be used for clinical pharmacokinetic studies of nicardipine hydrochloride in human plasma.