HPLC-ELSD法测定不同来源人工蛹虫草子实体中甘露醇的含量___

目的：建立HPLC-ELSD法测定人工蛹虫草子实体中甘露醇的含量。方法：采用氨基柱（4.6 mm×250 mm，5 μm），柱温25 ℃，进样量20 μL，以乙腈-水（82.5∶17.5）为流动相洗脱，流速0.5 mL•min-1 ELSD漂移管温度 90 ℃，载气（N2）流速 2.0 L•min-1，撞击器:关。结果：不同来源的人工蛹虫草子实体中甘露醇含量差别较大，其中以广东Ⅰ号厂家培育的含量最高（7.5%）。结论：本方法稳定、可靠，甘露醇可作为评价人工蛹虫草子实体质量的指标之一。

Objective:To establish a reliable and accurate HPLC-ELSD method for the determination of mannitol in cultured Cordyceps militaris L.Link by optimizing the extraction,separation,and analytical conditions.Method:Separation was achieved on a NH2 column (4.6 mm×250 mm，5 μm) with acetonitrile-water (82.5∶17.5) as mobile phase at 25 ℃ and a flow rate at 0.5 mL•min-1.The drift tube temperature of ELSD and nitrogen flow rate was set at 90 ℃,and 2.0 L•min-1,respectively，Impact:OFF.Results:Mannitol was found in 9 samples collected in different areas in China and the variety of mannitol was significant.The highest content (7.5%) was found in Guangdong province.Conclusion:The current method demonstrated good linearity,selectivity,precision,recovery,short analysis time,and was successfully used to determine the content of mannitol in cultured Cordyceps militaris growing in different areas of China.