采用固体复合酶测定果糖二磷酸钠含量的方法研究___

目的：建立固体复合酶活力测定方法及采用固体复合酶测定果糖二磷酸钠（FDP）含量的酶反应方法。方法：在固体复合酶作用下，通过测量还原型辅酶Ⅰ（NADH）在340 nm处吸收度的变化来测定果糖二磷酸的含量。结果：果糖二磷酸钠在0.1～0.6 mg•mL-1范围内线性关系良好（r=0.9997），注射用果糖二磷酸钠平均回收率为99.3%（n=9）；果糖二磷酸钠注射液平均回收率为99.0%（n=9）。结论：本法方法简便、准确，专属性强，可用于果糖二磷酸的含量测定。

Objective:To establish a method for the determination of enzymatic activity of solid compound enzyme and established a method of enzymatic reaction for the determination of sodium fructose diphosphate（FDP） by solid compound enzyme.Method:The solid compound enzyme was used in the determination of sodium fructose diphosphate by absorption change of NADH at 340 nm.Result:There was a good linear relationship of sodium fructose diphosphate within the range of 0.1-0.6 mg•mL-1 (r=0.9997).The average recovery of sodium fructose diphosphate for injection was 99.3%(n=9).The average recovery of sodium fructose diphosphate injection was 99.0%(n=9).Conclusion:The method was simple，accurate，specific and suitable for the determination of sodium fructose diphosphate.