HPLC测定当归补血片中黄芪甲苷的含量___

Objective:To establish a method for the determination of astragaloside Ⅳ in Danggui Buxue tablets.Methods:Astragaloside Ⅳ in Danggui Buxue tablets was determined by HPLC with pre-column derivatization,the derivatizing reagent were benzoyl chloride and pyridine.Column: Phenomenex Gemini C₁₈ column(50 mm×4.6 mm,5 μm);mobile phase:acetonitrile-0.1% triethylamine solution(98∶2) with the same gradient elution of 30 min at the flow rate of 1.0 mL·min^-1;detection wavelength:230 nm;column temperature:20 ℃;injection volume:10 μL. Results: The linear range of astragaloside Ⅳ was 0.074-0.925 μg(r=0.9999); The method had a good repeatability(RSD=0.17%) and average recovery(98.6%-99.9%) with RSD of 0.32%-0.95%. Conclusion: The method is accurate, specific, sensitive and reproducible, and preparation of the sample is simple.