LC-MS/MS方法鉴定中药制剂中掺入的7种西药成分___
Identification of seven chemical substances added into traditional Chinese medicine with LC-MS/MS
分类号：
出版年·卷·期（页码）：2009,29 (8):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的：采用LC-MS/MS联用技术，检查中药制剂中掺入的茶碱、醋酸泼尼松、地西泮、吡罗昔康、萘普生、吲哚美辛、双氯芬酸钠。方法：色谱柱为Phenomenex C18（250mm×4.6mm，5µm），柱温30℃，流动相为甲醇-甲酸盐缓冲溶液（取甲酸铵0.5g，加水2000 mL使溶解，用冰醋酸调pH值至3.86）（60：40），流速1mL·min-1，检测波长为240nm，电喷雾离子源（ESI），正离子模式，雾化气压力207kpa，干燥气温度350℃，流速10L·min-1，扫描范围100～600。结果：检出6批假药中掺入的茶碱、醋酸泼尼松、地西泮、吡罗昔康、萘普生、吲哚美辛、双氯芬酸钠。 结论： 建立的LC-MS/MS方法可同时鉴定掺入的上述西药成分。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: TodeployLC-MS/MSmultiple techniques for identify theophylline，prednisone acetate， diazepam，piroxicam，naproxen，indometacin，diclofenac sodium added into traditional Chinese medicine. Methods: The column was phenomenex C18（250mm×4.6mm，5µm）,and the temperature was set at 30℃,the mobile phase was methanol-ammonium formate buffer(0.5g ammonium formate dissolved in 2000mL water , adjust pH to 3.86 with acetate acid)(60:40),and the flow rate was 1mL·min-1,the detection wavelength was at 240nm,Electrospray ionization source(ESI)was applied and operated in positive ion mode, nebulizer pressure was 207kpa,drying gas temperature was at 350℃ and flow was 10L·min-1, the scanning range was from 100 to 600. Results: Foundseven above mentioned components in six counterfeit drugs. Conclusion: The LC-MS/MS methods established can be used to quantitatively determine the seven above mentioned components simultaneously.
-----参考文献：---------------------------------------------------------------------------------------
1 ChP(中国药典).2005.Vol Ⅱ(二部)：Appendix(附录)28
2 LING Xiao(凌霄).Chinese Journal of Pharmaceutical Analysis(药物分析杂志). 2007,27(12):1920
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