RP-HPLC法测定香加皮中异香草醛、杠柳毒苷和4-甲氧基水杨醛的含量___

摘要 目的：建立同时测定香加皮中异香草醛、杠柳毒苷和4-甲氧基水杨醛含量的方法。方法：采用RP-HPLC法，Hypersil ODS2 色谱柱（250 mm × 4.6 mm，5 μm，大连依利特） 流动相为乙腈（A）-0.02%磷酸水溶液（B）系统，梯度洗脱［0~10 min，85%B 10~40 min，85%B→70%B 40~60 min，70% B→50%B］ 流速：1.0 mL•min-1 检测波长为230 nm。结果：异香草醛、杠柳毒苷和4-甲氧基水杨醛进样浓度分别在1.65~13.7，5.91~49.2，4.97~99.4 μg•mL-1范围内与峰面积呈良好的线性关系(r分别为0.9996，0.9994，0.9996) 方法回收率(n=9)分别为101.7%，99.5%，99.9%。结论：该方法简便、准确，重复性好，适用于香加皮中异香草醛、杠柳毒苷和4-甲氧基水杨醛的定量分析。

Abstract Objective:To establish a method for simultaneous determination of isovanillin,periplocin and 4-methoxy salicylaldehyde in Cortex Periplocae.Methods:The analysis was performed on a Hypersil ODS2 column (250 mm 4.6 mm,5 μm).The mobile phase consisted of acetonitrile (A) and 0.02% aqueous phosphoric acid (B),with gradient program adopted as follows:0-10 min,85%B 10-40 min,85%B→70%B 40-60 min,70%→50%B.The flow rate was maintained at 1.0 mL•min-1 and the detection wavelength was set at 230 nm.Results:The linear response ranges were 1.65-13.7 μg•mL-1 for isovanillin (r=0.9996),5.91-49.2 μg•mL-1 for periplocin (r=0.9994) and 4.97-99.4 μg•mL-1 for 4-methoxy salicylaldehyde (r=0.9996).The recoveries (n=9) of these compounds were 101.7%,99.5% and 99.9%,respectively.Conclusion:The method is simple,accurate and reproducible for quality control of Cortex Periplocae.

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