双波长RP-HPLC法同时测定茵陈蒿中绿原酸、咖啡酸和对羟基苯乙酮的含量___
RP-HPLC simultaneous determination of chlorogenic acid，caffeic acid and 4-hydroxyacetophenone in herb of Artemisia capillaris Thunb. under different UV-vis wavelengths
分类号：
出版年·卷·期（页码）：2009,29 (6):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
摘要 目的：建立同时测定茵陈蒿中绿原酸、咖啡酸和对羟基苯乙酮含量的方法。方法：采用紫外-双波长同时检测的RP-HPLC法：C18柱(250 mm × 4.6 mm,5 μm )，流动相为乙腈-0.04%磷酸水溶液（9∶91），流速1.0 mL·min-1，检测波长：325 nm（绿原酸、咖啡酸） 275 nm（对羟基苯乙酮）。结果：绿原酸、咖啡酸和对羟基苯乙酮进样浓度分别在52.10~1.303×103 μg·mL-1(r=0.9999)、1.756~43.91 μg·mL-1 (r=0.9999)、0.6574~16.43 μg·mL-1 (r=0.9995)范围内与峰面积呈良好的线性关系(n=5) 方法回收率 (n=9) 分别为98.1%，98.6%，100.8%。结论：方法简便、快速、准确，重现性好，为茵陈蒿药材的质量控制提供依据。
-----英文摘要：---------------------------------------------------------------------------------------
Abstract Objective:To establish a method for the determination of chlorogenic acid，caffeic acid and 4-hydroxyacetophenone in herb of Artemisia capillaris Thunb.simultaneously under different UV wavelengths.Methods:An RP-HPLC method detected by different UV wavelengths—325 nm for chlorogenic acid and caffeic acid，275 nm for 4-hydroxyacetophenone respectively had been developed：The system consisting of a C18 column (250 mm × 4.6 mm,5 μm ) and a mixture of acetonitrile and 0.04% phosphate acid as the mobile phase was adopted The flow rate was 1.0 mL·min-1.Results:The linear response range was 52.10-1.303×103 μg·mL-1(r=0.9999)，1.756-43.91 μg·mL-1 (r=0.9999) and 0.6574-16.43 μg·mL-1 (r=0.9995)，respectively (n=5).The average recoveries (n=9) of chlorogenic acid，caffeic acid and 4-hydroxyacetophenone were 98.1%,98.6% and 100.8%,respectively.Conclusion:The assay demonstrates that the method has adequate accuracy and selectivity to measure the four chemical constituents in herb of Artemisia capillaris.
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