离子色谱法测定谷胱甘肽等含酰胺基药物中游离铵盐的研究___
HPLC ion chromatography determination of free ammonium salt in glutathione and other drugs containing amide
分类号：
出版年·卷·期（页码）：2009,29 (6):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
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摘要 目的：建立离子色谱法测定谷胱甘肽等含酰胺基药物中游离铵盐的含量。方法：采用IonPac CS12A色谱柱(250 mm×4 mm，8 μm)，IonPac CG12A预柱(50 mm×4 mm，8 μm)，以18 mmol· L-1 甲烷磺酸溶液为淋洗液，流速1.0 mL· min-1，电导检测器检测。结果：NH+4在0.203~4.07 μg·mL-1浓度范围内有良好的线性关系，r=0.9995 最低检测限为 0.025 ng 平均加样回收率在92.1%~100.6%之间。结论：本法简便、快速、准确，灵敏度高，重复性好，适用于含酰胺基药物中游离铵盐的质量控制。
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Abstract Objective：To establish an ion chromatography(IC) method for determination of free ammonium salt in glutathione and other drugs containing amide.Methods：A column of IonPac CS12A (250 mm×4 mm，8 μm) and a guard column of IonPac CG12A (50 mm×4 mm，8 μm),a eluent consisting of 18 mmol· L-1 methane-sulfonic with a flow rate of 1.0 mL·min-1,and a conductivity detector were adopted.Results:The linear range of the determination was 0.203-4.07 μg·mL-1,r=0.9995 The limit detection was 0.025 ng The average recovery was from 92.1% to 100.6%.Conclusion:The method is simple,rapid,accurate,reproducible and high sensitivity.It can be used for quality control of drugs containing amide group.
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