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期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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RP-HPLC法测定猪苓中麦角甾醇的含量___

RP-HPLC determination of ergosterol in Polyporus umbellatus(Pers.) Fries

分类号:
出版年·卷·期(页码):2009,29 (6):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

 
摘要目的:建立猪苓药材中麦角甾醇的RP-HPLC含量测定方法。方法:猪苓经甲醇超声提取后,用HPLC测定。采用Diamosil C18(250 mm×4.6 mm,5 μm)色谱柱,流动相为甲醇 流速为1.0 mL·min-1 检测波长为283 nm 柱温30 ℃。结果:麦角甾醇进样量在1.64~16.4 μg范围内与峰面积呈良好的线性关系(r=0.9997) 麦角甾醇的平均回收率(n=5)为98.9%,RSD为1.2%。结论:采用RP-HPLC测定猪苓药材中麦角甾醇的含量,该方法灵敏、快速、准确,可作为2010年版中国药典一部增订猪苓含量测定项。
 

-----英文摘要:---------------------------------------------------------------------------------------

Abstract Objective:To establish an HPLC method for the determination of ergosterol content in Polyporus umbellatus by RP-HPLC.Method:Polyporus umbellatus was extracted with methanol by ultrasound extraction and the chromatographic procedure was carried out with Diamosil C18 (250 mm×4.6 mm,5 μm) column,The mobile phase was 100% methanol at a flow of 1.0 mL·min-1 and the detection wavelength was 283 nm.The temperature of column was 30 ℃.Results:The linear range of ergosterol was 1.64-16.4 μg (r=0.9997).The average recovery(n=5) of ergosterol was 98.9% and RSD was 1.2%.Conclusion:The method is simple,accurate and reproducible can offer references for the quality control of Polyporus umbellatus.

-----参考文献:---------------------------------------------------------------------------------------
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