RP-HPLC法测定猪苓中麦角甾醇的含量___
RP-HPLC determination of ergosterol in Polyporus umbellatus(Pers.) Fries
分类号:
出版年·卷·期(页码):2009,29 (6):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------
摘要目的:建立猪苓药材中麦角甾醇的RP-HPLC含量测定方法。方法:猪苓经甲醇超声提取后,用HPLC测定。采用Diamosil C18(250 mm×4.6 mm,5 μm)色谱柱,流动相为甲醇 流速为1.0 mL·min-1 检测波长为283 nm 柱温30 ℃。结果:麦角甾醇进样量在1.64~16.4 μg范围内与峰面积呈良好的线性关系(r=0.9997) 麦角甾醇的平均回收率(n=5)为98.9%,RSD为1.2%。结论:采用RP-HPLC测定猪苓药材中麦角甾醇的含量,该方法灵敏、快速、准确,可作为2010年版中国药典一部增订猪苓含量测定项。
-----英文摘要:---------------------------------------------------------------------------------------
Abstract Objective:To establish an HPLC method for the determination of ergosterol content in Polyporus umbellatus by RP-HPLC.Method:Polyporus umbellatus was extracted with methanol by ultrasound extraction and the chromatographic procedure was carried out with Diamosil C18 (250 mm×4.6 mm,5 μm) column,The mobile phase was 100% methanol at a flow of 1.0 mL·min-1 and the detection wavelength was 283 nm.The temperature of column was 30 ℃.Results:The linear range of ergosterol was 1.64-16.4 μg (r=0.9997).The average recovery(n=5) of ergosterol was 98.9% and RSD was 1.2%.Conclusion:The method is simple,accurate and reproducible can offer references for the quality control of Polyporus umbellatus.
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1ChP (中国药典).2005.Vol Ⅰ(一部):222
2ZHOU Wen-pu(周文璞).Pharmacognosy research of traditional Chinese medicine Polyporus umbellatus (Pers.) Fries (中药猪苓的生药学研究).China J Chin Mater Med(中国中药杂志),1990,15(8):458
3Miyazaki T,Oikawa N.Studies on fungal polysaccharide Ⅻ.Water-soluble polysaccharide of Grifora umbellata (Fr.).Chem Pharm Bull,1973,21(11):2545
4Lu W,Adachi I,Kano K,et al.Platelet aggregation potentiators from Cho-Rei.Chem Pharm Bull,1985,33(11):5083
5Ohsawa T,Yukawa M,Takao C,et al.Studies on constituents of fruit Body of Polyporus umbellatus and their cytotoxic activity.Chem Pharm Bull,1992,40(1):143
6Yuan D,Mori J,Komatsu KI,et al.An anti-aldosteronic diuretic component (drain dampness) in Polyporus Sclerotium.Biol Pharm Bull,2004,27(6):867
7Sun Y,Yasukawa K.New anti-inflammatory ergostane-type ecdysteroids from the sclerotium of Polyporus umbellatus.Bioorg Med Chem Lett,2008,18:3417
8Inaoka Y,Shakuya A,Fukazawa H,et al.Studies on active substances in herbs used for hair treatment.I.Effects of herb extracts on hair growth and isolation of an active substance from Polyporus umbellatus F..Chem Pharm Bull,1994,42(3):530
9Krzyczkowski W,Malinowska E,Suchocki P,et al.Isolation and quantitative determination of ergosterol peroxide in various edible mushroom species.Food Chem,2009,113:351
10Price MJ,Worth GK.The occurrence of ergosta-4,6,8(14),22-tetraen-3-one in several Fungi.Aust J Chem,1974,27:2505
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