动力学荧光法测定痕量吲哚美辛___

摘要目的：建立测定痕量吲哚美辛的动力学荧光测定新方法，并应用于吲哚美辛的测定。方法：在缓冲溶液中，吲哚美辛对Cu（Ⅱ）催化H2O2氧化罗丹明B使其荧光猝灭有抑制作用，据此，建立了动力学荧光测定痕量吲哚美辛的新方法。结果：在0.32~1.43 ng·mLa12345a浓度范围内，吲哚美辛与荧光强度有良好的线性关系，方法的检出限为0.25 ng·mLa12345a。结论：该方法实用、灵敏，准确度高，检测限低，重现性好，已成功地用于胶囊和尿液中的吲哚美辛的测定。

AbstractObjective:To develop a new method of kinetic fluorimetric for the determination of trace indometacin.Method:In buffer solution,indometacin (IND) has an inhibitory effection on the reaction of H2O2 oxidation of rhodamine B catalyzd by Cu（Ⅱ）,based on this reaction,a new sensitive kinetic fluorimetric determination of trace IND method has been proposed.Results:The result show that in the range of 0.32-1.43 ng·mLa12345a,there is linear relationship between the concentration of IND and its fluorescence intensity,with the detection limit of 0.25 ng·mLa12345a.Conclusion:This method is convenient,sensitive,and suitable,has been successfully applied to the determination of IND in capsule and body urine.

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