HPLC法测定女贞子药材中女贞苷的含量___

摘要 目的：建立女贞子药材的含量测定方法。方法：采用高效液相色谱法测定女贞苷的含量。使用Alltima C₁₈色谱柱(4.6 mm×250 mm，5 μm)，流动相为乙腈(A)-水(B)，梯度洗脱[0～40 min，15% A→40% A］，流速1.0 mL·min^-1，检测波长230 nm。结果：女贞苷线性范围50～1000 μg·mL^-1(r=0.9999)；平均回收率(n=5)为99.01%(RSD=0.17%)。不同来源10批女贞子药材含量测定结果表明，女贞子药材中女贞苷含量为0.24%～3.06%，综合分析10批样品含量测定结果，建议女贞子药材中含女贞苷应不低于1.7%。结论：本方法简便、准确，重复性好，专属性强，可作为女贞子药材质量控制方法。

关键词：女贞子；女贞苷；高效液相色谱法；含量测定

 

 

 

Abstract   Objective:To develop a quantitative method for the determination of nuezhenide in Fructus Ligustri Lucidi.Method:An HPLC method was applied to separate and quantify nuezhenide.The samples were separated by an Alltima C₁₈ column(4.6 mm×250 mm,5 μm) with acetonitrile(A)-water(B) as mobile phase[0-40 min，15% A→40% A].The flow rate was 1.0 mL·min^-1,and the detection wavelength was set at 230 nm.Results：The linear range of nuezhenide was 50-1000 μg·mL^-1(r=0.9999),and the average recovery(n=5) was 99.01%(RSD=0.17%).As the results shown,the content of nuezhenide in Fructus Ligustri Lucidi was 0.24%-3.06% from 10 different habitats.Our study suggests that the content of nuezhenide in Fructus Ligustri Lucidi should not be less than 1.7%.Conclusion：The developed method is sensitive,rapid,accurate and specific.And it can be used to control the quality of Fructus Ligustri Lucidi.

Key words：Fructus Ligustri Lucidi;nuezhenide;HPLC;quantitative determination