HPCE法测定通塞脉微丸中甘草苷、甘草素和阿魏酸的含量___

摘要 目的：研究HPCE法同时测定通塞脉微丸中甘草苷、甘草素和阿魏酸的含量。方法：以葛根素为内标，40 mmol·L^-1四氢硼钠（pH 9.18）-甲醇（90∶10）为运行缓冲液。采用未涂层弹性石英毛细管柱（75 μm×50 cm，有效长度42.5 cm），分离电压20 kV；进样压力5 kPa，进样1.5 s；毛细管温度：25℃；二极管阵列检测，测定波长λ_{s=320 nm,λR=380 nm。结果：上述条件下，通塞脉微丸中甘草苷，甘草素和阿魏酸在25 min内得到很好地分离，加样回收率分别为99.3%，98.7%和100.2%；RSD分别为3.2%，2.6%和1.7%。结论：本方法具有良好的精密度和回收率，可作为通塞脉微丸质量控制的方法。}

Abstract Objective:To develop an HPCE method for the determination of liquiritin，liquiritigenin and ferulic acid in Tongsaimai pellets.Methods: Puerarin was used as an internal standard.The buffer was composed of 40 mmol·L^-1 borax (pH 9.18) -methanol (90∶10).CE was performed on a 75 μm×50 cm (effective length is 42.5 cm)fused silica capillary.Separation voltage was 20 kV，sampling pressure was 5 kPa，sampling time was 1.5 s and the temperature was maintained at 25 ℃.Results: liquiritin，liquiritigenin and ferulic acid were successfully separated within 25 min，the recoveries of them were 99.3%，98.7% and 100.2% and RSDs were 3.2%，2.6% and 1.7%，respectively. Conclusion: This method is convenient, rapid and accurate. It can be used for the determination of liquiritin，liquiritigenin and ferulic acid in Tongsaimai pellets.

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