RP-HPLC法同时测定小儿柴桂退热口服液中7个成分的含量

目的：建立同时测定小儿柴桂退热口服液中没食子酸、葛根素、芍药苷、黄芩苷、柴胡皂苷a、柴胡皂苷d和桂皮醛的含量测定方法。方法：采用反相高效液相色谱法，色谱柱为Thermo ODS C₁₈（250 mm×4.6 mm，5 μm），以乙腈-0.1%磷酸水溶液为流动相，梯度洗脱，流速1.0 mL·min^-1，检测波长254 nm（没食子酸、葛根素、芍药苷）、278 nm（黄芩苷）、210 nm （柴胡皂苷a、柴胡皂苷d）、290 nm（桂皮醛），柱温25℃，进样量20 μL。结果：没食子酸、葛根素、芍药苷、黄芩苷、柴胡皂苷a、柴胡皂苷d和桂皮醛检测质量浓度的线性范围分别为0.007 8～0.093 3、0.154 4～1.853 2、0.031 3～0.375 6、0.070 4～0.845 0、0.043 4～0.521 2、0.023 2～0.278 9、0.013 1～0.157 7 mg·mL^-1（r为0.999 4～0.999 9）；平均加样回收率分别为101.1%、100.3%、98.8%、101.2%、98.3%、99.9%、99.4%（RSD<2.0%，n=6）。5批小儿柴桂退热口服液样品中测得的上述7个成分平均含量分别为0.200～0.221、4.146～4.200、0.818～0.856、1.698～1.754、1.086～1.236、0.586～0.732、0.084～0.320 mg·mL^-1。结论：该方法操作简便，结果准确，重复性好，可用于小儿柴桂退热口服液的质量控制。

Objective: To develop a method for simultaneous determination of gallic acid,puerarin,paeoniflorin,baicali,saikosaponin a,saikosaponin d and cinnamaldehyde in Xiao' er Chaigui Tuire oral liquid by RP-HPLC. Methods: The analysis was carried out on a Thermo ODS C₁₈(250 mm×4.6 mm,5 μm)column with the mobile phase consisting of acetonitrile and 0.1% phosphoric acid in a gradient mode at a flow rate of 1.0 mL·min^-1. The detection wavelengths were set at 254 nm for gallic acid,puerarin and paeoniflorin,278 nm for baicali,210 nm for saikosaponin a,saikosaponin d and 290 nm for cinnamaldehyde. The column temperature was set at 25℃ and the injection volume was 20 μL. Results: The linear rangs were 0.007 8-0.093 3, 0.154 4-1.853 2,0.031 3-0.375 6,0.070 4-0.845 0,0.043 4-0.521 2,0.023 2-0.278 9,0.013 1-0.157 7 mg·mL^-1 for gallic acid,puerarin,paeoniflorin,baicali,saikosaponin a,saikosaponin d and cinnamaldehyde,respectively(r=0.999 4-0.999 9). Average recoveries were 101.1%,100.3%,98.8%,101.2%,98.3%,99.9% and 99.4%,respectively(RSD<2.0%,n=6). The average contents of the above 7 components in 5 samples were 0.200-0.221,4.146-4.200,0.818-0.856,1.698-1.754,1.086-1.236,0.586-0.732 and 0.084 -0.320 mg·mL^-1,respectively.Conclusions: The established method is simple,accurate and reproducible. It can be used for quality control of Xiao'er Chaigui Tuire oral liquid.

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