近红外光谱法快速测定益智不同药用部位的水溶性浸出物含量

目的： 建立快速测定益智仁和益智壳中水溶性浸出物含量的近红外光谱模型。方法： 通过近红外光谱结合化学计量学建立水溶性浸出物的定量分析模型。结果： 益智仁样品的水溶性浸出物含量在12.84%~31.33%之间。益智仁中水溶性浸出物的近红外光谱定量分析模型的内部交叉验证决定系数（R²）为0.984 79，校正均方差（RMSEC）为0.005 54，预测均方差（RMSEP）为0.006 67。益智壳样品的水溶性浸出物含量在24.65%~38.42%。益智壳近红外分析模型的R²为0.980 33，RMSEC为0.012 4，RMSEP为0.013 1。结论： 该方法建立的模型可用于益智仁和益智壳中水溶性浸出物的定量分析，并可为益智的质量评价体系的建立提供基础。

Objective: To establish a near-infrared spectroscopy model for the rapid determination of water-soluble extracts in seed and shell of Alpinia oxyphylla Miq.Methods: Quantitative analysis model of water-soluble extracts were established by near-infrared spectroscopy combined with chemometrics.Results: The contents of water-soluble extract in Alpinia oxyphylla seed were between 12.84% and 31.33%.The correlation coefficients(R2),the root-mean-square error of calibration(RMSEC)and the root-mean-square error of predication(RMSEP)of the NIRS model for water-soluble extract in Alpinia oxyphylla seed were 0.984 79,0.005 54 and 0.006 67 respectively.The contents of water-soluble extract of Alpinia oxyphylla in shell were between 24.65% and 38.42%.The R²,RMSEC and RMSEP of the NIRS model for water-soluble extract in Alpinia oxyphylla shell were 0.980 33,0.012 4 and 0.013 1 respectively.Conclusion: The established model can be used for quantitative analysis of water-soluble extracts in seed and shell of Alpinia oxyphylla,and also provides basis for the establishment of quality evaluation system of Alpinia oxyphylla.

[1] 中华人民共和国药典2015年版.一部[S].2015:291 ChP 2015.Vol Ⅰ[S].2015:291
[2] CHUN KS,PARK KK,LEE J,et al.Inhibition of mouse skin tumor promotion by anti-inflammatory diarylheptanoids derived from Alpinia oxyphylla Miquel(Zingiberaceae)[J].Oncol Res,2002,13(1):37
[3] SHIN TY,WON JH,KIM HM,et al.Effect of Alpinia oxyphylla fruit extract on compound 48/80-induced anaphylactic reactions[J].Am J Chin Med,2001,29(2):293
[4] YAMAHARA J,LI YH,TAMAI Y.Anti-ulcer effect in rats of bitter cardamon constituents[J].Chem Pharm Bull,1990,38(11):3053
[5] XIE Y,MAN X,NI Y,et al.Alpinia oxyphylla Miq.extract prevents diabetes in mice by modulating gut microbiota[J].J Diabetes Res,2018,2018(12):1
[6] XIE Y,MAN X,DAN L,et al.Anti-diabetic effect of Alpinia oxyphylla extract on 57BL/KsJ db-/db-mice[J].Exp Ther Med,2017,13(4):1321
[7] 马俊俏,吴勇,周俊璇,等.益智仁挥发油对东莨菪碱致小鼠学习记忆障碍的改善作用研究[J].中国药房,2018,29(22):3074 MA JQ,WU Y,ZHOU JX,et al.Study on improvement effects of volatile oil from the fruit of Alpinia oxyphylla on scopolamine-induced learning and memory impairment in mice[J].China Pharm,2018,29(22):3074
[8] HAN Z,CAI S,ZHANG X,et al.Development of predictive models for total phenolics and free p-coumaric acid contents in barley grain by near-infrared spectroscopy[J].Food Chem,2017,227:342
[9] PRIETO N,PAWLUCZYK O,DUGAN M,et al.A review of the principles and applications of near-infrared spectroscopy to characterize meat,fat,and meat products[J].App Spectrosc,2017,71(7):1403
[10] CORRO-HERRERA VA,GóMEZ-RODRíGUEZ J,HAYWARD-JONES PM,et al.In-situ monitoring of Saccharomyces cerevisiae ITV01 bioethanol process using near-infrared spectroscopy NIRS and chemometrics[J].Biotechnol Prog,2016,32(2):510
[11] DOUGLAS RK,NAWAR S,ALAMAR MC,et al.Rapid prediction of total petroleum hydrocarbons concentration in contaminated soil using vis-NIR spectroscopy and regression techniques[J].Sci Total Environ,2018,616-617:147
[12] ZHANG G,LI P,ZHANG W,et al.Analysis of multiple soybean phytonutrients by near-infrared reflectance spectroscopy[J].Anal Bioanal Chem,2017,409(7):1
[13] TEIXEIRA KSS,da CRUZ FONSECA SG,de MOURA LCB,et al.Use of chemometrics to compare NIR and HPLC for the simultaneous determination of drug levels in fixed-dose combination tablets employed in tuberculosis treatment[J].J Pharm Biomed Anal,2017,149:557
[14] DING G,LI B,HAN Y,et al.A rapid integrated bioactivity evaluation system based on near-infrared spectroscopy for quality control of Flos Chrysanthemi[J].J Pharm Biomed Anal,2016,131:391
[15] 赖秀娣,贾灿潮,林晓菁,等.利用NIRS技术快速测定制何首乌中游离蒽醌含量[J].药物分析杂志,2016,36(12):2215 LAI XD,JIA CC,LIN XJ,et al.Rapid content determination of free anthraquinones in Polygoni Multiflori Radix Praeparata by using NIRS[J].Chin J Pharm Anal,2016,36(12):2215